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Droplet creation is a crucial but challenging subject in microfluidics. Depending on the application, the objective is to produce droplets of a particular size. Two immiscible phases are used in microfluidics to create droplets. These are:

Microfluidic systems typically consist of a microfluidic chip, a fluid handling device, and tubing for producing droplets. The droplet generation can then be seen by connecting this equipment to a computer and a microscope.

Droplet-based microfluidic systems have already entered laboratories, and currently, it is possible to create (monodisperse) droplets that range in size from femto to nanoliters. Therefore, all kinds of experimental possibilities are accessible. For instance, the compartmentalization and surface-to-volume ratio are crucial in single-cell studies and digital PCR.

The case study below demonstrates the feasibility of droplet creation. Bartels’ partner, microfluidic ChipShop, provided the mp6 micropump, a pressure sensor, reagents, fluidic accessories, and a Droplet Generator Chip.

Image Credit: Bartels Mikrotechnik GmbH

The graphic provides info on how the case study’s system is configured. There are several critical details that must be taken into account to guarantee optimal performance.

This case study provides an in-depth view of the workings of this great use for microfluidics.

Droplet generation is crucial to medical technology. The analysis and sorting of single cells are where this technique is most intriguing. The video below demonstrates how to create a compact, adaptable system that does not require a compressed air line, unlike many other systems. This is made possible by a microfluidic ChipShop chip and the mp6 micropump.

Video Credit: Bartels Mikrotechnik GmbH

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